MODEL OF ANTIGEN-ANTIBODY COMPETITIVE REACTION IN INHIBITION ZONE

Factors affecting the antigen-antibody reaction.

Types of weak interactions According to quantum mechanics, all chemical bonds are based on electrostatic forces. A list of types of weak bonds is shown in table I, together with their strength (energy). Van der Waals forces are the weakest, but they are able to attract all kinds of molecules. Hydrogen or iondipole bonds require oppositely charged atoms. Hydrogen bonds are very important in aque...

The antibody-antigen reaction: a visual observation.

Biochemical Study of Cellular Antigen-antibody Reaction in Tissue Culture

The correlation between morphological and biochemical changes produced by the antigen-antibody reaction was studied in cultures of tissue monocytes taken from sensitized animals. The cells were grown under conditions which allowed collection of samples from the culture fluid as well as microscopic observation. Introduction of the antigen into the culture medium causes rapid release of a proteas...

Detection of antigen-antibody reaction using a fluorescent probe and its application to homogeneous competitive-type immunoassay for insulin.

The interaction of bovine insulin with anti-human insulin antibody (mAb) was examined using a fluorescent probe. The fluorescence intensity of fluoresceinthiocarbamyl (FTC)-insulin was increased by adding mAb, and the increase was saturated at 53% at a molar ratio of FTC-insulin to mAb of 2.0. Based on the change in fluorescence intensity, a standard curve of the homogeneous competitive-type im...

Antigen-antibody Reaction: Nature of Complex Initiating Delayed Hypersensitivity.

Two homologous lightly coupled dinitrophenyl conjugates of poly-L-lysine of differing average molecular sizes were compared with regard to their abilities to elicit in guinea pigs specific delayed hypersensitivity skin reactions, passive cutaneous anaphylaxis, and active Arthus reactions. Equal concentrations by weight (but not equimolar concentrations) of the two conjugates elicited equally in...